Effect of Short-Chain Fatty Acids on the Yield of 2,3-Butanediol by Saccharomyces cerevisiae W141: The Synergistic Effect of Acetic Acid and Dissolved Oxygen

As a platform chemical, 2,3-butanediol (2,3-BDO) has been widely used in various industrial fields. To improve the yield of 2,3-BDO produced by Saccharomyces cerevisiae W141, this paper explored effects exogenous short-chain fatty acids (SCFAs) as well synergistic acetic acid and dissolved oxygen content on from perspective physiological metabolism. The results indicated that different SCFAs had production 2,3-BDO, higher or lower concentrations were not conducive to generation 2,3-BDO. However, exogenically adding 1.0 g/L significantly increased expression level bdh1, key gene synthesis (p < 0.05). In addition, concentration 4.52 mg/L was proven be optimal condition for production. When g/L, respectively, maximum 3.25 ± 0.03 which 66.59% than S. W141 alone. These provide methodological guidance cerevisiae.